On Fri, 05 Sep 2008 14:42:31 -0400, Jon Mcleod
wrote:
Mr. Clark,
The control is another steak is similar size from the same grocery
packaging, placed in a second apparatus that is not powered.
The idea is that a certain frequencies of low-voltage e-fields inhibit
cellular mitosis.
The professor saw an article in Science magazine about treating cancer
with these fields, and decided it would be a nice lab activity for his
students to observe other effects with this type of field.
http://www.rife.de/files/disruption_...eplication.pdf
Hi Jon,
Let's examine the hypothesis of "low-voltage e-fields" in light of the
source:
In Vitro Experimental Set Up. Cultures were grown in standard
culture dishes (4-well cell culture chambers; SN 138121; Nalge
Nunc International). The TTFields were generated by pairs of
15-mm-long, completely insulated wires (P/N K-30–1000; VT
Corporation; outer diameter, 0.5 mm; ethylene tetrafluoroethylene
insulation thickness, 0.125 mm; dielectric breakdown,
1800 V/mil) fixed to the bottom of each dish at a distance of 1 mm
from each other. The wires were connected to an oscillator
(GFG8219A; Instek) and a high-voltage amplifier (A303; A. A. Lab
Systems Ltd.) that generated the required sine-wave signals
(range, 300-800 V).
This would equate to an average of 5,500V/cM or roughly 275kV across
the chest of a convict. History reveals that this prisoner would be
specially prepared with conductive paste and electrodes that were NOT
insulated (such as specified in your source). Thus we have two
differences:
1. Frequency (100KHz vs. 60 Hz);
2. Insulation;
3. Application (in situ vs. in vitro).
Since generating an e-field in "meat" or "plant material" is not so
easy, this is part of the assignment. We need to figure it out and then
fully describe it in our methods section. The leaf people are using
high-voltage insulated wires. With "meat", which is larger, I don't
think this will work. I am wondering if I can use a low-voltage direct
connection.
You would then be changing the conditions from testing fields to
testing conduction. Ask yourself, if faced with the prospects of
sitting in the electric chair, would you care to amend the conditions
to include insulative pads at all points that formerly contacted the
seat? Your rational answer would suggest you already know you are
redefining expectations.
My problem is that our group is weak on electromagnetism. They have
been floundering, and now I am floundering with them. I have some
ideas, but ideas are best vetted through those with PRACTICAL
experience, which you guys seem to have. I have also wasted almost 2
weeks by sitting in the wrong group. OK I am an idiot in terms of BOTH
electromagnetism and reading directions.
Bottom line: I need to generate a 1V/cm field across a t-bone steak.
Merit or no merit (this is college). Does anyone have any place I might
look on line, or any book I might buy, or any advice on how I might
accomplish this?
You stick to the source if you want a good grade. To replicate the
experiment, you have to conform exactly to the conditions, but not
necessarily the means.
It follows from shallow reading (speaking of my own efforts, not
yours) that there is not much current flowing (otherwise this would
immediately place us in the chair with the convict). This is to your
advantage. You use the function generator you have, apply it to what
is called a step-up transformer to obtain the voltage specified; and
you come very close to the original conditions. Your function
generator should have enough power to both step-up and supply the low
current (you will have to confirm this through other means). You will
have to use a small portion of meat because this is an e-field
experiment that is measured in volt/meter. Choosing a T-Bone
exacerbates your voltage problem, and making a direct connection
invalidates it.
Almost any insulated wire will perform adequately, the source is
simply providing information for you to choose a suitable alternative,
it is not necessary to find the exact reel of wire from a specific
manufacturer. Google for the topic "100 KHz voltage transformer" and
you will find a design that shows you how to construct one within an
hour of obtaining the cheap components. It is merely a matter of
ratios (how much voltage out of your function generator, and how much
do you need?). This, of course, requires you to have a voltmeter that
measures voltage at that frequency.
Go to the EE department and visit their second year circuits lab.
73's
Richard Clark, KB7QHC