The changes you have made should not knock the alignment off. I generally
like to fix the problems before alignment. Alignment is generally "gilding
the lilly".

It is not uncommon to acquire a radio that is off because of alignment.
But, even if that is the case, it often means that there was another
unsolved problem which precipitated alignment.

You can usually tell when an alignment has been attempted by someone not
qualified as they leave their calling cards on the adjustment points.

Once the basics are corrected, you can usually use a signal applied at
various stages to check stage amplification. If the radio is working on the
lower bands, that is a good start. Look for switch contacts, or high
frequency oscillator failure (I have had that with flat 6C4 tubes) or even
the mixer tube. Then, carefully check the alignment - being sure to follow
the alignment manual and always makr your starting point before any
adjustment.

The previous comments have been good.

Colin K7FM